Downregulation of IL-6 and TNF-α Expression with Mesenchymal Stem Cells Therapy in Allergic Rhinitis Rats Models

  • Lia Restimulia Departement of Doctoral Degree Program, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia. Departement of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas
  • Dian Andriani Ratna Dewi Faculty of Medicine, Indonesian Defense University, Indonesia Peace and Security Center (IPSC) Sentul Bogor West Java, Indonesia
  • Mohammad Ariq Nazar
  • Risky Chandra Satria Irawan Postgraduated in Biomedical Sciences, Departement of Medicine, Sultan Agung Islamic University, Semarang, Central Java 50112, Indonesia
  • Shabrina Syifa Ghaissani Graduate School of Biology, Department of Biology, Faculty of Science and Data Analytics, Institute Technology of Sepuluh Nopember, Surabaya, East Java, Indonesia
  • Erlina Haryono
Keywords: Mesenchymal Stem Cells, TNF-α, IL-6, Rhinitis Allergy

Abstract

Rhinitis is an inflammatory that characterized by nasal symptoms due to the condition of the nasal mucosa that trigerred by an interaction between environmental allergens and immunoglobulin (Ig)E. It is driven by host factors, infection, pathogens and various inflammatory pathways such as TNF-α that released in allergic responses from both mast cells and macrophages through IgE-dependent mechanisms. Secretome hypoxia mesenchymal stem cells (SH-MSCs) contain anti-inflammatory soluble molecules were able to improve the conditions of endothelial damage, inflammation and oxidative stress by decreasing levels of IL-6, TNF-α, ROS and increasing eNOS. This study will investigate the effect of SH-MSCs at a dose of 300 µL on TNF- and IL-6 expression in rat model ovalbumin-induced rhinitis in vivo. Thirty male Wistar rats were randomly divided into 3 groups: control, treatment and sham group that administrated through intramuscular injection. The results of this study found that SH-MSCs can downregulate the inflammatory cytokines TNF-α and IL-6 in rat model ovalbumin-induced rhinitis in vivo

Published
2022-04-22
How to Cite
Restimulia, L., Dewi, D. A. R., Nazar, M. A., Irawan, R. C. S., Ghaissani, S. S., & Haryono, E. (2022). Downregulation of IL-6 and TNF-α Expression with Mesenchymal Stem Cells Therapy in Allergic Rhinitis Rats Models. International Journal of Cell and Biomedical Science, 1(1), 27-34. Retrieved from https://cbsjournal.com/cbs/article/view/13
Section
Articles