The Effect of Hypoxia on the Soluble Molecules of Human Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSCs)

Effect of hypoxia on MSCs secretome

  • Agus Widyatmoko
  • Iffan Alif Stem Cell and Cancer Research (SCCR), Faculty of Medicine, Sultan Agung Islamic University (UNISSULA), Semarang, Indonesia
  • Risky Candra Satria Irawan
  • Frigi Eko Handoyo
  • Husni Ahmad Sidiq
Keywords: UC-MSCs, hypoxia, secretome, wound healing, survival


Background: Umbilical cord-derived stem cells (UC-MSCs) are essential cell sources for cell-based therapies in regenerative medicine. Hypoxia is a key element of the stem cell niche and crucial for the preservation of UC-MSCs properties. The normal growth conditions for UC-MSCs are under atmospheric oxygen concentrations of 20–21%. However, the administration of UC-MSCs in inflammatory conditions provides oxygen-deficient environments. Thus, treating UC-MSCs with low oxygen exposure provides them with more survival and recovery potential.

Objective:  In this study, we assessed the impact of hypoxia incubation for 12 h on the UC-MSCs proteome.

Methods: UC-MSCs were isolated from UC patients regarding informed consent. At passage 5, in 80% confluent, UC-MSCs were incubated in 5% O2 for 12 h. The morphology of UC-MSCs was assessed using a microscope. The level of FGF-2, FGF-8, TNF-α, and HSP-70 were analyzed using ELISA.

Results: Hypoxic condition could change their morphology and enhance the cellular density compared to normoxic conditions in vitro. The level of FGF-2, FGF-8, TNF-α, and HSP-70 were significantly increased after the hypoxic condition of UC-MSCs compared to normoxia.

Conclusion: Our findings suggest that the hypoxic condition was able to induce survival capacity and soluble molecules secreted by UC-MSCs.



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How to Cite
Widyatmoko, A., Alif, I., Irawan, R. C. S., Handoyo, F. E., & Sidiq, H. A. (2023). The Effect of Hypoxia on the Soluble Molecules of Human Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSCs). International Journal of Cell and Biomedical Science, 1(3), 102-108.