International Journal of Cell and Biomedical Science

The Effect of X-Ray Radiation to IL-10 Levels in Secretome Mesenchymal Stem Cells Cosmeceutical Product

Arini Dewi Antari — 2023-09-20

Background: X-ray radiation has been widely used in the pharmaceutical industry because it regenerates and repairs damaged tissues. Objective: In this study, we evaluate the effect of X-ray radiation on the secretome cosmeutical product. Methods: We conducted interleukin 10 (IL-10) analysis by ELISA in each product sample after exposure to X-ray radiation. Results: The levels of IL-10 in each sample were significantly lower than those in the control samples. Moreover, the level of IL-10 in the product samples was significantly higher than that of the control sample. Conclusion: In conclusion, exposure to radiation during shipping or storage of skin care products can potentially damage the proteins in the products by inducing the production of reactive oxygen species (ROS) and decreasing the treatment effectiveness.

Effect of Time Transport on Mesenchymal Stem Cell Surface Markers: Unveiling the Influence of Cellular Translocation on Cellular Phenotype

Hendi Wicaksono — 2023-09-20

Mesenchymal stem cells (MSCs) hold great promise in regenerative medicine due to their ability to differentiate into multiple cell types and promote tissue repair. However, the effect of transportation time on the surface markers of MSCs remains understudied. This study investigated the impact of transportation time on MSC surface markers, specifically CD73, CD90, CD105, and hematopoietic lineage markers. MSCs were isolated from human umbilical cords and cultured. Flow cytometry analysis confirmed the expression of MSC surface markers. The MSCs were then subjected to simulated transportation for different time periods ranging from 0 to 24 hours at 2-8^oC. After transportation, flow cytometry was used to analyze the expression of surface markers. The results showed that prolonged transportation time led to a decrease in the expression levels of CD73, CD90, and CD105, which are important markers for maintaining MSC functionality. Additionally, there was an increase in hematopoietic lineage marker expression. These findings suggest that transportation time can compromise the therapeutic potential of MSCs. Further investigation is needed to understand the underlying mechanisms responsible for the observed changes in surface marker expression. Optimization strategies, such as improved temperature control and protective agents, should be considered to mitigate the negative effects of prolonged transportation time. In conclusion, this study highlights the importance of considering transportation time and its impact on MSC surface markers in cellular therapy protocols. Understanding and addressing these effects are crucial for ensuring the quality and effectiveness of MSC-based therapies.

Secretome MSCs restore α-Smooth Muscle Actin Protein Tissue Expression in Croton Oil-Induced Hemorrhoid Rats

M. Hidayat Budi Kusumo — 2023-09-20

Background: Hemorrhoidal disease, a prevalent and distressing condition affecting a significant number of the population, presents a considerable challenge in both clinical management and patient quality of life. Secretome mesenchymal stem cell hypoxia (S-HMSCs) is involved in accelerated remodeling and regeneration of wound tissue, including hemorrhoids, through anti-inflammatory and anti-fibrotic molecules paracrine activities. Objective: This study aims to investigate the effect of Secretome Hypoxia MSCs (S-HMSCs) in restoring α-smooth muscle actin (α-SMA) expression in croton oil-induced hemorrhoid rats. Material and Methods: An experimental study with a post-test-only control group design was used in this study. Croton oil was administrated for inducting hemorrhoidal disease. A total of 24 male Wistar rats were divided into four groups (n=6); Sham (Healthy group); Untreated (Croton oil+NaCl 300 µL) Croton oil+S-HMSCs 150); Secretome 150 µL (Croton oil+S-HM SCs 150 µL) and Secretome 300 µL (Croton oil+S-HMSCs 300 µL). S-HMSCs were injected intraperitoneally every week for up to 4 weeks. All animals were scarified and the rectal tissue was collected for α-SMA immunohistochemical staining analysis. Results: After hemorrhoid induction, α-SMA was expressed 20% higher than Sham group, furthermore, administration of 150 µL and 300 µL of S-HMSCs may decreased by 15% and 20% α-SMA expression compared to the Untreated group, expression in croton oil-induced hemorrhoid rats.

Comparison of Two Tangential Flow Filtration Methods in Isolating CD63+/CD9+ Mesenchymal Stem Cell Exosome

Agung Putra — 2023-09-20

Background: Extracellular vesicles, particularly CD63+/CD9+ Mesenchymal Stem Cell Exosome (MSC-Exo), have emerged as crucial mediators of intercellular communication and potential therapeutic agents, including regenerative medicine and immunomodulation. However, the precise isolation and purification of MSC exosomes pose critical challenges. Tangential Flow Filtration (TFF) has gained recognition as an efficient exosome isolation method, offering scalability and versatility. In this study, we address the pressing need for standardized exosome isolation methods by comparing two distinct TFF-based protocols for isolating CD63+/CD9+ MSC exosomes based on filter size pore order. Methods: MSC-Exo were conducted from the Stem Cell and Cancer Research Laboratory (SCCR Indonesia), which were then processed through TFF using different filter sizes and orders. There are two filtration methods compared, first, MSC-Exo was filtered with 1000-5-500-300-100-50-10-5 filter order. Second procedure, MSC-Exo was filtered using 1000-500-300-100-50-10-5 filter order. Result: Flow cytometry analysis revealed variations in the percentage of CD63+/CD9+ in the MSC-Exo based on filter order. The results indicate that the choice of filter order significantly influences the size range with the highest concentration of CD63+/CD9+ MSC-Exo. Conclusion: This research underscores the importance of optimizing TFF-based isolation methods for CD63+/CD9+ MSC exosomes, especially in the order of filter pore size.

Intracavernous Stem Cell Injection for Erectile Dysfunction: Safety and Efficacy Review

Ahmad Sulaiman Lubis — 2023-09-20

Background: Erectile dysfunction is a common condition in men characterized by the inability to maintain an erectile state. Current treatment using phosphodiesterase-5 inhibitors treatment may leave significant side effects and effectivity problems in some patients. The use of stem cell transplantation in erectile dysfunction has been emerging as a replacement therapy for erectile dysfunction. The effectiveness of stem cell therapy for the treatment of erectile dysfunction has been investigated in several animal studies and one clinical trial in humans. The intracavernous injection is one of the methods to deliver stem cell treatment for erectile dysfunction besides intravenous and intraperitoneal. It was believed to improve erectile function by promoting cavernosal tissue regeneration, vascularization, and smooth muscle relaxation. However, the intracavernous injection of stem cells for erectile dysfunction treatment in clinical settings requires further investigation since its long-term efficacy and safety concerns regarding swelling, priapism, and fibrous plaque. Objective: This review aims to summarize the available evidence on the safety and efficacy of intracavernous stem cell injection for erectile dysfunction treatment in clinical studies only from PubMed. Conclusion: In conclusion, while the available evidence suggests that intracavernous stem cell injection is considered safe and effective for the treatment of erectile dysfunction, further large-scale clinical studies are needed to determine its long-term safety, efficacy, and optimal dosing regimens.