Hypoxia-Preconditioned MSCs for Enhanced Bone Regeneration in Ovine Fracture Case

Okky Ardianto; Berlian  Ramadhanti

doi:10.59278/cbs.v4i12.78

Okky Ardianto Vaccinology and Immunotherapeutics Program, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya. 60115. Indonesia
Berlian Ramadhanti Vaccinology and Immunotherapeutics Program, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya. 60115. Indonesia

DOI: https://doi.org/10.59278/cbs.v4i12.78

Keywords: Secretome, Hypoxia, Mesenchymal Stem Cells, Fracture, Wound healing

Abstract

Background: Bone fracture is a common clinical issue that occurs in humans and animals. The fracture-wound healing process may have a variable duration since several conditions interfere with tissue regeneration. Mesenchymal stem cells (MSCs)-based therapy offers regenerative potential. Objective: This study aimed to evaluate the therapeutic effects of hypoxia umbilical cord mesenchymal stem cell (UC-MSC) secretome on fracture wound healing in sheep. Methods: A one-year-old male thin-tailed sheep (Ovis aries) weighing 25 kg had a fracture in the hindlimb digits. The sheep was treated with hypoxia MSC secretome injection thoroughly at the wound area. MSCs were isolated from the umbilical cord and cultured. The cell culture was conditioned in a hypoxic environment (1 – 5% O2). Results: The results showed that secretome application significantly accelerated the fracture wound healing process. Conclusion: This finding indicates that the hypoxia UC-MSC secretome has the potential as a non-cellular regenerative approach in animal orthopaedic cases.

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