Trisindoline 5 Compound Inhibits Human Breast Cancer Stem Cell Formation by Downregulating the BCL-2 Gene Expression

  • Muhammad Fatoni
  • Yofinta I Salsabila
  • Awik P.D. Nurhayati Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Surabaya 60111, Indonesia
  • Shabrina Syifa Ghaissani
  • Mardi Santoso
  • Fahimah Martak Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Surabaya 60111, Indonesia
Keywords: trisindoline 5, BCL-2, Breast Cancer Stem Cells

Abstract

Background:  Breast cancer cell's growth and survival are supported by breast cancer stem cells (bCSCs) mammosphere formation. bCSCs represent a subpopulation of undifferentiated cancer cells which associated with self-renewing abilities, tumor initiation, drug resistance, and metastasis. Overexpression of the B Cell Lymphoma 2 (BCL-2) family in many tumor cells contributes to apoptosis resistance. Trisindoline is an indole trimer alkaloid natural compound that provides a cytotoxic effect on cancer cells. A new modification of trisindoline has been synthesized into trisindoline 5 in 2021. Objective: This study purposed to investigate the effect of trisindoline 5 compounds against BCL-2 gene expression in bCSCs in vitro. Methods: The bCSCs MDA-MB-231 were divided into control and treatment groups which were further analyzed in gene expression using the qPCR Livak method. Results: Based on gene expression analysis, the results showed that trisindoline 5 may decrease the expression of BCL-2 in MDA-MB-231 cells. Conclusion: This study concludes that trisindoline 5 could downregulate the antiapoptotic BCL-2 gene expression in bCSCs in vitro.

Published
2022-09-30
How to Cite
Fatoni, M., Salsabila, Y. I., Nurhayati, A. P., Ghaissani, S. S., Santoso, M., & Martak, F. (2022). Trisindoline 5 Compound Inhibits Human Breast Cancer Stem Cell Formation by Downregulating the BCL-2 Gene Expression. International Journal of Cell and Biomedical Science, 1(2), 65-69. Retrieved from https://cbsjournal.com/cbs/article/view/19
Section
Articles

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