The Effect of Celery Extract on Caspase-3 and TNF-α Gene Expression in Lead Poisoning-Induced Renal Injury in Rats
Keywords:
Apium graveolens, TNF-α, caspase 3, lead acetate poisoning
Abstract
Background To determine the effect of Apium graveolens L (Celery) extract in the TNF-α and caspase-3 gene expression on the lead poisoning-induced renal injury rats’ model. Methods This study is experimental research with post test only control group design. Twenty male Wistar rats were randomly allocated into four groups (n=5). The control group was orally administrated with distillate water. The second group received lead acetate 200mg/kg body weight orally for 14 days, the third group were orally administered lead acetate 200 mg lead acetate/kg body weight and vitamin E 50IU/kg body weight for 14 days. The fourth group was administrated with leas acetate like second group and celery extract 300mg/kg body weight for 14 days. The TNF-a and caspase-3 gene expression was analyses under qRT-PCR. Results The phytochemical analysis of APE indicated the presence of alkaloids, flavonoids, tannins, saponins, and steroids. Leads acetate increased the TNF-α (3.87±0.09) and caspase-3 (7.95±0.23) gene expression. The celery extract was significantly decrease in the TNF-α (3.13±0.34) and caspase-3 (2.48±1.23) gene expression. Conclusion: In conclusion, the presence of celery extract inhibited the renal injury-induced lead acetate toxicity by inhibition of TNF-α proinflammation protein and caspase-3 proapoptosis protein
References
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28. Wani AL, Ara A, Usmani JA. Lead toxicity: A review. Interdiscip Toxicol. 2015;8(2):55-64. doi:10.1515/intox-2015-0009
29. Marianti A, Mahatmanti FW. Synergetic effect of chitosan and vitamin C on the oxidative enzyme status in rat exposed to lead acetate. Acta Sci Biol Sci. 2018;40(1):1-8. doi:10.4025/actascibiolsci.v40i1.41869
30. AL-Megrin WA, Soliman D, Kassab RB, Metwally DM, Dina DM, El-Khadragy MF. Coenzyme Q10 Activates the Antioxidant Machinery and Inhibits the Inflammatory and Apoptotic Cascades Against Lead Acetate-Induced Renal Injury in Rats. Front Physiol. 2020;11(February):1-13. doi:10.3389/fphys.2020.00064
31. Brunetti C, di Ferdinando M, Fini A, Pollastri S, Tattini M. Flavonoids as antioxidants and developmental regulators: Relative significance in plants and humans. Int J Mol Sci. 2013;14(2):3540-3555. doi:10.3390/ijms14023540
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35. Suzery M, Cahyono B, Amalina ND. Citrus sinensis (L) peels extract inhibits metastasis of breast cancer cells by targeting the downregulation matrix metalloproteinases-9. Open Access Maced J Med Sci. 2021;9(B):464-469. doi:10.3889/oamjms.2021.6072
36. Amalina ND, Suzery M, Cahyono B, ... Mengungkap Potensi Metabolit Sekunder Tanaman Herbal Indonesia untuk Menghentikan Metastasis Kanker Payudara: Pendekatan in-silico. Indonesian Journal of …. 2020;9(3).
37. Paramita DA, Hermansyah D, Paramita DA, Amalina ND. Regulation of p53 and survivin by Curcuma longa extract to caspase-3 dependent apoptosis in triple negative breast cancer cells. Med Glas. 2022;19(2):189-196. doi:10.17392/1453-22
38. Hao J, Lou P, Han Y, et al. Ultraviolet-B Irradiation Increases Antioxidant Capacity of Pakchoi (Brassica rapa L.) by Inducing Flavonoid Biosynthesis. Plants. 2022;11(6). doi:10.3390/plants11060766
39. Bajpai VK, Baek KH, Kang SC. Antioxidant and free radical scavenging activities of taxoquinone, a diterpenoid isolated from Metasequoia glyptostroboides. South African Journal of Botany. 2017;111:93-98. doi:10.1016/j.sajb.2017.03.004
40. Furuichi K, Kokubo S, Hara A, et al. Fas Ligand Has a Greater Impact than TNF-α on Apoptosis and Inflammation in Ischemic Acute Kidney Injury. Nephron Extra. 2012;2(1):27-38. doi:10.1159/000335533
41. Aly O, Abouelfadl DM, Shaker OG, Hegazy GA, Fayez AM, Zaki HH. Hepatoprotective effect of Moringa oleifera extract on TNF-α and TGF-β expression in acetaminophen-induced liver fibrosis in rats. Egyptian Journal of Medical Human Genetics. 2020;21(1). doi:10.1186/s43042-020-00106-z
42. Putra A, Antari AD, Kustiyah AR, et al. Mesenchymal stem cells accelerate liver regeneration in acute liver failure animal model. Biomedical Research and Therapy. 2018;5(11):2802-2810. doi:10.15419/bmrat.v5i11.498
43. Hamra NF, Putra A, Tjipta A, Amalina ND, Nasihun T. Hypoxia mesenchymal stem cells accelerate wound closure improvement by controlling α-smooth muscle actin expression in the full-thickness animal model. Open Access Maced J Med Sci. 2021;9:35-41. doi:10.3889/oamjms.2021.5537
2. Flora G, Gupta D, Tiwari A. Toxicity of lead: A review with recent updates. Interdiscip Toxicol. 2012;5(2):47-58. doi:10.2478/v10102-012-0009-2
3. Liu CM, Sun YZ, Sun JM, Ma JQ, Cheng C. Protective role of quercetin against lead-induced inflammatory response in rat kidney through the ROS-mediated MAPKs and NF-κB pathway. Biochim Biophys Acta Gen Subj. 2012;1820(10):1693-1703. doi:10.1016/j.bbagen.2012.06.011
4. Patra RC, Rautray AK, Swarup D. Oxidative stress in lead and cadmium toxicity and its amelioration. Vet Med Int. 2011;2011. doi:10.4061/2011/457327
5. Sudjarwo S, Sudjarwo G, Koerniasari K. Protective effect of curcumin on lead acetate-induced testicular toxicity in Wistar rats. Res Pharm Sci. 2017;12(5):381-390. doi:10.4103/1735-5362.213983
6. Abdel Moneim AE, Dkhil MA, Al-Quraishy S. The protective effect of flaxseed oil on lead acetate-induced renal toxicity in rats. J Hazard Mater. 2011;194:250-255. doi:10.1016/j.jhazmat.2011.07.097
7. Lowry J, (MD Clinics D of CP and MTTCMH and. ORAL CHELATION THERAPY FOR PATIENTS WITH LEAD POISONING Jennifer A . Lowry , MD Division of Clinical Pharmacology and Medical Toxicology The Children ’ s Mercy Hospitals and Clinics Kansas City , MO 64108. Blood. 2010;(December).
8. Mursiti S, Amalina ND, Marianti A. Inhibition of breast cancer cell development using Citrus maxima extract through increasing levels of Reactive Oxygen Species (ROS). J Phys Conf Ser. 2021;1918(5). doi:10.1088/1742-6596/1918/5/052005
9. Amalina ND, Wahyuni S, Harjito. Cytotoxic effects of the synthesized Citrus aurantium peels extract nanoparticles against MDA-MB-231 breast cancer cells. J Phys Conf Ser. 2021;1918(3). doi:10.1088/1742-6596/1918/3/032006
10. Suzery M, Cahyono B, Amalina ND. Citrus sinensis (L) peels extract inhibits metastasis of breast cancer cells by targeting the downregulation matrix metalloproteinases-9. Open Access Maced J Med Sci. 2021;9(B):464-469. doi:10.3889/oamjms.2021.6072
11. Kooti W, Daraei N. A Review of the Antioxidant Activity of Celery (Apium graveolens L). J Evid Based Complementary Altern Med. 2017;22(4):1029-1034. doi:10.1177/2156587217717415
12. Al-Attar AM. Vitamin E attenuates liver injury induced by exposure to lead, mercury, cadmium and copper in albino mice. Saudi J Biol Sci. 2011;18(4):395-401. doi:10.1016/j.sjbs.2011.07.004
13. Marianti A, Christijanti W, Mursiti S. Effect of Gamma Co60-irradiated chitosan and vitamin e towards Pb acetate cytotoxicity on rat kidney. J Phys Conf Ser. 2021;1918(5):1-6. doi:10.1088/1742-6596/1918/5/052008
14. Suzery M, Cahyono B, Amalina ND. Antiproliferative and apoptosis effect of hyptolide from Hyptis pectinata ( L .) Poit on human breast cancer cells. 2020;10(02):1-6. doi:10.7324/JAPS.2020.102001
15. Amalina ND, Suzey M, Cahyono B. Cytotoxic Activity of Hyptis pectinata Extracts on MCF-7 Human Breast Cancer Cells. Indonesian Journal of Cancer Chemoprevention. 2020;02(February):1-6.
16. Cahyono B, Suzery M, Amalina ND, Bima DN. Synthesis and antibacterial activity of epoxide from hyptolide ( Hyptis pectinata ( L .) Poit ) against Gram-positive and Gram- negative bacteria. 2020;10(12):13-22. doi:10.7324/JAPS.2020.101202
17. Hermansyah D, Putra A, Munir D, Lelo A, Amalina ND, Alif I. Synergistic Effect of Curcuma longa Extract in Combination with Phyllanthus niruri Extract in Regulating Annexin A2 , Epidermal Growth Factor Receptor , Matrix Metalloproteinases , and Pyruvate Kinase M1 / 2 Signaling Pathway on Breast Cancer Stem Cell. 2021;9:271-285.
18. Fernanda MAHF, Andriani RD, Estulenggani Z, Kusumo GG. Identification and Determination of Total Flavonoids in Ethanol Extract of Old and Young Angsana Leaves (Pterocarpus indicus Willd.) Using Visible Spectrophotometry. In: Scitepress; 2019:541-544. doi:10.5220/0007546605410544
19. Y R, Y I, M.S I. Comparative Phyto-Constituents Analysis from the Root Bark and Root Core Extractives of Cassia ferruginea (Schrad D. C) Plant. Scholars Journal of Agriculture and Veterinary Sciences. 2016;3(4):275-283. doi:10.21276/sjavs.2016.3.4.1
20. Sri Sulasmi E, Saptasari M, Mawaddah K, Ama Zulfia F. Tannin identification of 4 species pterydophyta from baluran national park. In: Journal of Physics: Conference Series. Vol 1241. Institute of Physics Publishing; 2019. doi:10.1088/1742-6596/1241/1/012002
21. Adu JK, Amengor CDK, Kabiri N, Orman E, Patamia SAG, Okrah BK. Validation of a Simple and Robust Liebermann-Burchard Colorimetric Method for the Assay of Cholesterol in Selected Milk Products in Ghana. Int J Food Sci. 2019;2019. doi:10.1155/2019/9045938
22. Pękal A, Pyrzynska K. Evaluation of Aluminium Complexation Reaction for Flavonoid Content Assay. Food Anal Methods. 2014;7(9):1776-1782. doi:10.1007/s12161-014-9814-x
23. Amalina ND, Wahyuni S, Harjito. Cytotoxic effects of the synthesized Citrus aurantium peels extract nanoparticles against MDA-MB-231 breast cancer cells. J Phys Conf Ser. 2021;1918(3):032006. doi:10.1088/1742-6596/1918/3/032006
24. Prasad Raju Borelli D, Raju P, Tirumanyam Sri Padmavati Mahila Visvavidyalayam M, Sushma Nannepaga Sri Padmavati Mahila Visvavidyalayam J. IDENTIFICATION OF BIOACTIVE COMPOUNDS BY FTIR ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF CLITORIA TERNATEA LEAF AND FLOWER EXTRACTS I Am Working on Rare Earth Ions Doped Glasses and Glad Ceramics View Project Thermal and Optical Properties of Glasses Doped with Rare Earths and Nanoparticles View Project.; 2014. www.iajpr.com
25. Farkhondeh T, Boskabady M, Koohi M, Sadeghi-Hashjin G, Moin M. The Effect of Lead Exposure on Selected Blood Inflammatory Biomarkers in Guinea pigs. Cardiovascular & Hematological Disorders-Drug Targets. 2013;13(1):45-49. doi:10.2174/1871529x11313010005
26. Trachtman L, Doucet HJ, Hazel M. Lead poisoning. J La State Med Soc. 2021;136(9):28-32.
27. Lidsky TI, Schneider JS. Lead neurotoxicity in children: Basic mechanisms and clinical correlates. Brain. 2003;126(1):5-19. doi:10.1093/brain/awg014
28. Wani AL, Ara A, Usmani JA. Lead toxicity: A review. Interdiscip Toxicol. 2015;8(2):55-64. doi:10.1515/intox-2015-0009
29. Marianti A, Mahatmanti FW. Synergetic effect of chitosan and vitamin C on the oxidative enzyme status in rat exposed to lead acetate. Acta Sci Biol Sci. 2018;40(1):1-8. doi:10.4025/actascibiolsci.v40i1.41869
30. AL-Megrin WA, Soliman D, Kassab RB, Metwally DM, Dina DM, El-Khadragy MF. Coenzyme Q10 Activates the Antioxidant Machinery and Inhibits the Inflammatory and Apoptotic Cascades Against Lead Acetate-Induced Renal Injury in Rats. Front Physiol. 2020;11(February):1-13. doi:10.3389/fphys.2020.00064
31. Brunetti C, di Ferdinando M, Fini A, Pollastri S, Tattini M. Flavonoids as antioxidants and developmental regulators: Relative significance in plants and humans. Int J Mol Sci. 2013;14(2):3540-3555. doi:10.3390/ijms14023540
32. Li P, Jia J, Zhang D, Xie J, Xu X, Wei D. In vitro and in vivo antioxidant activities of a flavonoid isolated from celery (Apium graveolens L. var. dulce). Food Funct. 2014;5(1):50-56. doi:10.1039/c3fo60273g
33. Banjarnahor SDS, Artanti N. Antioxidant properties of flavonoids. Medical Journal of Indonesia. 2014;23(4):239-244. doi:10.13181/mji.v23i4.1015
34. Yong-Bing X, Gui-Lin C, Ming-Quan G. Antioxidant and anti-inflammatory activities of the crude extracts of moringa oleifera from kenya and their correlations with flavonoids. Antioxidants. 2019;8(8). doi:10.3390/antiox8080296
35. Suzery M, Cahyono B, Amalina ND. Citrus sinensis (L) peels extract inhibits metastasis of breast cancer cells by targeting the downregulation matrix metalloproteinases-9. Open Access Maced J Med Sci. 2021;9(B):464-469. doi:10.3889/oamjms.2021.6072
36. Amalina ND, Suzery M, Cahyono B, ... Mengungkap Potensi Metabolit Sekunder Tanaman Herbal Indonesia untuk Menghentikan Metastasis Kanker Payudara: Pendekatan in-silico. Indonesian Journal of …. 2020;9(3).
37. Paramita DA, Hermansyah D, Paramita DA, Amalina ND. Regulation of p53 and survivin by Curcuma longa extract to caspase-3 dependent apoptosis in triple negative breast cancer cells. Med Glas. 2022;19(2):189-196. doi:10.17392/1453-22
38. Hao J, Lou P, Han Y, et al. Ultraviolet-B Irradiation Increases Antioxidant Capacity of Pakchoi (Brassica rapa L.) by Inducing Flavonoid Biosynthesis. Plants. 2022;11(6). doi:10.3390/plants11060766
39. Bajpai VK, Baek KH, Kang SC. Antioxidant and free radical scavenging activities of taxoquinone, a diterpenoid isolated from Metasequoia glyptostroboides. South African Journal of Botany. 2017;111:93-98. doi:10.1016/j.sajb.2017.03.004
40. Furuichi K, Kokubo S, Hara A, et al. Fas Ligand Has a Greater Impact than TNF-α on Apoptosis and Inflammation in Ischemic Acute Kidney Injury. Nephron Extra. 2012;2(1):27-38. doi:10.1159/000335533
41. Aly O, Abouelfadl DM, Shaker OG, Hegazy GA, Fayez AM, Zaki HH. Hepatoprotective effect of Moringa oleifera extract on TNF-α and TGF-β expression in acetaminophen-induced liver fibrosis in rats. Egyptian Journal of Medical Human Genetics. 2020;21(1). doi:10.1186/s43042-020-00106-z
42. Putra A, Antari AD, Kustiyah AR, et al. Mesenchymal stem cells accelerate liver regeneration in acute liver failure animal model. Biomedical Research and Therapy. 2018;5(11):2802-2810. doi:10.15419/bmrat.v5i11.498
43. Hamra NF, Putra A, Tjipta A, Amalina ND, Nasihun T. Hypoxia mesenchymal stem cells accelerate wound closure improvement by controlling α-smooth muscle actin expression in the full-thickness animal model. Open Access Maced J Med Sci. 2021;9:35-41. doi:10.3889/oamjms.2021.5537
Published
2024-12-10
How to Cite
Purwaningsih, H., Sumarawati, T., Chodidjah, C., Putra, A., Priyantini, S., Fasitasari, M., Ibrahim, S., & Amalina, N. D. (2024). The Effect of Celery Extract on Caspase-3 and TNF-α Gene Expression in Lead Poisoning-Induced Renal Injury in Rats. International Journal of Cell and Biomedical Science, 2(6), 192-200. https://doi.org/10.59278/cbs.v2i6.42
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