Topical Gel of Mesenchymal Stem Cell-Conditioned Medium-induced Serum Injury Accelerates Wound Healing in Skin Excision Tissue

  • Mukti Arja Berlian RSPAU dr.S. Hardjolukito, Yogyakarta, Indonesia.
  • Iffan Alif Stem Cell and Cancer Research (SCCR), Faculty of Medicine, Sultan Agung Islamic University (UNISSULA), Semarang, Indonesia
  • Prasetyowati Subchan
  • Frigi Eko Handoyo Biomedical Postgraduate Student, Faculty of Medicine, Sultan Agung Islamic University (UNISSULA), Semarang, Indonesia
  • Sofian Azalia Husain Stem Cell and Cancer Research (SCCR) Indonesia, Semarang, Indonesia
  • Husni Ahmad Sidiq Stem Cell and Cancer Research (SCCR) Indonesia, Semarang, Indonesia
  • Dyken Dwi Arlinda
  • Resanti Adityani
Keywords: skin excision tissue, platelet derived growth factor, umbilical cord mesenchymal stem cell-conditioned medium, vascular endothelial growth factor, wound healing

Abstract

Introduction: Umbilical cord-derived mesenchymal stem cells (UC-MSCs) accelerating wound closure by increasing VEGF and PDGF level leading to re-epithelialization, cell infiltration, and angiogenesis. It has been found that MSC-conditioned medium (MSC-CM) can enhance migration of fibroblasts in scratch assays. However, the effect of MSC-CM-induced serum injury (MSC-CM-S) formulated in gel to accelerate wound healing remains unclear. This study aims to evaluate the effect of several doses of topical gel of MSC-CM-S in accelerating wound healing. Methods: The MSCs were cultured medium-supplemented serum injury of wounded rat (8:1) to get MSC-CM-S. The topical gel of MSC-CM-S was made by base gel supplemented with MSC-CM-S.  Eighteen Wistar rats were randomly assigned into control (C) and treatment groups (T1, T2). Groups were received serum-free medium gel (C), 25 µl MSC-CM-S in topical gel (T1), 50 µl MSC-CM-S in topical gel (T2), twice daily for 9 days. PDGF and VEGF level and fibroblast density were measured by ELISA and HE staining at day 3 and 6, respectively. Results: This study showed that there was significant increase of VEGF and PDGF level along with a significant increase of fibroblast density at day 3 and 6. The T2 showed optimum enhancement level of VEGF, PDGF and fibroblast density. Conclusion: Topical gel of MSC-CM-S was effective to accelerate wound closure by enhancing PDGF and VEGF level in full-thickness skin defect rats.

Published
2022-04-22
How to Cite
Berlian, M. A., Alif, I., Subchan, P., Handoyo, F. E., Husain, S. A., Husni Ahmad Sidiq, Arlinda, D. D., & Adityani, R. (2022). Topical Gel of Mesenchymal Stem Cell-Conditioned Medium-induced Serum Injury Accelerates Wound Healing in Skin Excision Tissue. International Journal of Cell and Biomedical Science, 1(1), 10-17. Retrieved from https://cbsjournal.com/cbs/article/view/12
Section
Articles